Bioprocessing:Article Title: The nsp3 Macrodomain Promotes Virulence in Mice with Coronavirus-Induced Encephalitis
Article Snippet: Cells were analyzed using a FACSVerse flow cytometer (BD Biosciences, Mountain View, CA). .. The following monoclonal antibodies were used for these studies: CD45-phycoerythrin (PE)-Cy7 (30-F11; BD Biosciences), CD11b-peridinin chlorophyll protein (Percp)-Cy5.5 (M1/70; BD Biosciences), Ly6C-allophycocyanin (APC) (AL-21; BD Biosciences), Ly6G-PE (1A8; BD Biosciences), F4/80-PE (BM8; eBioscience), and CD11c-eFluor 450 (N418; eBioscience). .. All flow cytometry data were analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
FACS:Article Title: SWAP-70 restricts spontaneous maturation of dendritic cells.
Article Snippet: .. Inflammatory cytokine production Table I. Abs for FACS, Western blotting, and functional assays used in this study Ag Conjugate Clone/Origin Company FACS CD11c eFluor 450, APC N418 eBioscience CD11c FITC HL3 BD Biosciences CD45.1 APC A20 BioLegend CD45.2 PerCP-Cy5.5 104 eBioscience CD86 (B7-2) APC GL1 eBioscience CD4 PE L3T4 BD Biosciences CD8a APC 53-6.7 eBioscience CD24 PE M1/69 BD Biosciences CD11b PE-Cy7 M1/70 eBioscience MHCII eFluor 450 M5/114.15.2 eBioscience MHCI FITC AF6-88.5 BD Biosciences ICAM-1 Unlabeled Human Fc-chimeric R&D Systems Anti human Fc FITC rabbit The Jackson Laboratory Western blotting GAPDH Unlabeled 6C5 Santa Cruz Biotechnology RhoA Unlabeled 119 Santa Cruz Biotechnology Active-b-catenin Unlabeled 8E7 Millipore b-Catenin Unlabeled Rabbit Sigma-Aldrich E-cadherin Unlabeled, AF 488 DECMA-1, rat eBioscience SMC3 Unlabeled Rabbit Lab self-made Anti-mouse HRP Donkey Jackson ImmunoResearch Anti-rabbit HRP Goat Jackson ImmunoResesarch Functional assays CD11b (aM) Unlabeled M1/70 eBioscience CD18 (b2) Unlabeled M18/2 eBioscience VCAM Unlabeled 429 eBioscience at U niv of Sydney E B S L ibrn/1359861 on February 1, 2014 http://w w w .jim m unol.org/ D ow nloaded from in wt and Swap-702/2 BMDCs without LPS activation was below detectable levels (data not shown). ..
Western Blot:Article Title: SWAP-70 restricts spontaneous maturation of dendritic cells.
Article Snippet: .. Inflammatory cytokine production Table I. Abs for FACS, Western blotting, and functional assays used in this study Ag Conjugate Clone/Origin Company FACS CD11c eFluor 450, APC N418 eBioscience CD11c FITC HL3 BD Biosciences CD45.1 APC A20 BioLegend CD45.2 PerCP-Cy5.5 104 eBioscience CD86 (B7-2) APC GL1 eBioscience CD4 PE L3T4 BD Biosciences CD8a APC 53-6.7 eBioscience CD24 PE M1/69 BD Biosciences CD11b PE-Cy7 M1/70 eBioscience MHCII eFluor 450 M5/114.15.2 eBioscience MHCI FITC AF6-88.5 BD Biosciences ICAM-1 Unlabeled Human Fc-chimeric R&D Systems Anti human Fc FITC rabbit The Jackson Laboratory Western blotting GAPDH Unlabeled 6C5 Santa Cruz Biotechnology RhoA Unlabeled 119 Santa Cruz Biotechnology Active-b-catenin Unlabeled 8E7 Millipore b-Catenin Unlabeled Rabbit Sigma-Aldrich E-cadherin Unlabeled, AF 488 DECMA-1, rat eBioscience SMC3 Unlabeled Rabbit Lab self-made Anti-mouse HRP Donkey Jackson ImmunoResearch Anti-rabbit HRP Goat Jackson ImmunoResesarch Functional assays CD11b (aM) Unlabeled M1/70 eBioscience CD18 (b2) Unlabeled M18/2 eBioscience VCAM Unlabeled 429 eBioscience at U niv of Sydney E B S L ibrn/1359861 on February 1, 2014 http://w w w .jim m unol.org/ D ow nloaded from in wt and Swap-702/2 BMDCs without LPS activation was below detectable levels (data not shown). ..
Functional Assay:Article Title: SWAP-70 restricts spontaneous maturation of dendritic cells.
Article Snippet: .. Inflammatory cytokine production Table I. Abs for FACS, Western blotting, and functional assays used in this study Ag Conjugate Clone/Origin Company FACS CD11c eFluor 450, APC N418 eBioscience CD11c FITC HL3 BD Biosciences CD45.1 APC A20 BioLegend CD45.2 PerCP-Cy5.5 104 eBioscience CD86 (B7-2) APC GL1 eBioscience CD4 PE L3T4 BD Biosciences CD8a APC 53-6.7 eBioscience CD24 PE M1/69 BD Biosciences CD11b PE-Cy7 M1/70 eBioscience MHCII eFluor 450 M5/114.15.2 eBioscience MHCI FITC AF6-88.5 BD Biosciences ICAM-1 Unlabeled Human Fc-chimeric R&D Systems Anti human Fc FITC rabbit The Jackson Laboratory Western blotting GAPDH Unlabeled 6C5 Santa Cruz Biotechnology RhoA Unlabeled 119 Santa Cruz Biotechnology Active-b-catenin Unlabeled 8E7 Millipore b-Catenin Unlabeled Rabbit Sigma-Aldrich E-cadherin Unlabeled, AF 488 DECMA-1, rat eBioscience SMC3 Unlabeled Rabbit Lab self-made Anti-mouse HRP Donkey Jackson ImmunoResearch Anti-rabbit HRP Goat Jackson ImmunoResesarch Functional assays CD11b (aM) Unlabeled M1/70 eBioscience CD18 (b2) Unlabeled M18/2 eBioscience VCAM Unlabeled 429 eBioscience at U niv of Sydney E B S L ibrn/1359861 on February 1, 2014 http://w w w .jim m unol.org/ D ow nloaded from in wt and Swap-702/2 BMDCs without LPS activation was below detectable levels (data not shown). ..
Activation Assay:Article Title: SWAP-70 restricts spontaneous maturation of dendritic cells.
Article Snippet: .. Inflammatory cytokine production Table I. Abs for FACS, Western blotting, and functional assays used in this study Ag Conjugate Clone/Origin Company FACS CD11c eFluor 450, APC N418 eBioscience CD11c FITC HL3 BD Biosciences CD45.1 APC A20 BioLegend CD45.2 PerCP-Cy5.5 104 eBioscience CD86 (B7-2) APC GL1 eBioscience CD4 PE L3T4 BD Biosciences CD8a APC 53-6.7 eBioscience CD24 PE M1/69 BD Biosciences CD11b PE-Cy7 M1/70 eBioscience MHCII eFluor 450 M5/114.15.2 eBioscience MHCI FITC AF6-88.5 BD Biosciences ICAM-1 Unlabeled Human Fc-chimeric R&D Systems Anti human Fc FITC rabbit The Jackson Laboratory Western blotting GAPDH Unlabeled 6C5 Santa Cruz Biotechnology RhoA Unlabeled 119 Santa Cruz Biotechnology Active-b-catenin Unlabeled 8E7 Millipore b-Catenin Unlabeled Rabbit Sigma-Aldrich E-cadherin Unlabeled, AF 488 DECMA-1, rat eBioscience SMC3 Unlabeled Rabbit Lab self-made Anti-mouse HRP Donkey Jackson ImmunoResearch Anti-rabbit HRP Goat Jackson ImmunoResesarch Functional assays CD11b (aM) Unlabeled M1/70 eBioscience CD18 (b2) Unlabeled M18/2 eBioscience VCAM Unlabeled 429 eBioscience at U niv of Sydney E B S L ibrn/1359861 on February 1, 2014 http://w w w .jim m unol.org/ D ow nloaded from in wt and Swap-702/2 BMDCs without LPS activation was below detectable levels (data not shown). ..
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